Why is it recommended to have a 40% 60% gc content?

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Cristobal Farrell asked a question: Why is it recommended to have a 40% 60% gc content?
Asked By: Cristobal Farrell
Date created: Wed, Jun 23, 2021 7:39 PM
Date updated: Tue, Aug 9, 2022 3:49 AM

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Top best answers to the question «Why is it recommended to have a 40% 60% gc content»

Aim for the GC content to be between 40 and 60% with the 3' of a primer ending in G or C to promote binding. This is known as a GC Clamp. The G and C bases have stronger hydrogen bonding and help with the stability of the primer.

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